Reporter

Part:BBa_J119063

Designed by: Michel Conn   Group: Eckdahl Lab   (2012-01-31)


pT7-RBS-GFP-TT-pLacI-RBS-T7 RNAP (mutant 2)

This construct consists of a GFP reporter with a T7 promoter. The T7RNAP has a frameshift mutation inserted at the beginning of the gene. The mutation consists of an 11 base oligonucleotide.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1030
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1063
    Illegal BsaI.rc site found at 694
    Illegal BsaI.rc site found at 1014


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Categories
Parameters
None